recombinant human metalloproteinase mmp  (R&D Systems)

Journal: Bioactive Materials

Article Title: Mechanically sensitized hydrogel microspheres trigger membrane receptor switch for cartilage repair

doi: 10.1016/j.bioactmat.2026.03.017

Figure Lengend Snippet: Efficacy of mechanically sensitized OBNC microspheres in the treatment of osteoarthritis. A) Diagram of OBNC microspheres self-adaptive filling of a cartilage injury site to promote cartilage repair and regeneration. B) Representative images of H&E staining. C) OARSI scores of articular cartilage in each group. D) Representative images of safranin O-fast green staining. E) Relative glycosaminoglycan (GAG) content in each group. F) Representative sections showing TUNEL staining for apoptotic cells. G) Quantification of TUNEL-positive cells. H) Representative sections showing Collagen II staining for chondrocytes. I) Relative collagen II expression in each group. J) Representative sections showing MMP-13 staining of chondrocytes. K) Relative MMP-13 expression in each group. (ns: non-significant, ∗ P < 0.05, ∗∗ P < 0.01).

Article Snippet: Antigen retrieval was performed by microwaving in citrate buffer for 10 min. Endogenous peroxidase activity was blocked by incubating the sections with 3% hydrogen peroxide for 10 min, and non-specific binding was blocked with 3% BSA for 1 h. The sections were incubated overnight at 4 °C with primary antibodies to Collagen II and MMP-13 (ServiceBio, China), washed, and incubated for 1 h with HRP-labeled secondary antibodies (ServiceBio, China).

Techniques: Staining, TUNEL Assay, Expressing

Journal: Non-coding RNA Research

Article Title: Hsa_circ_0101645 contributes to excessive autophagy and apoptosis in intervertebral disc degeneration by acting as a miR-1304-5p sponge modulating BNIP3 expression

doi: 10.1016/j.ncrna.2025.11.007

Figure Lengend Snippet: Hsa_circ_0101645 accelerating the IVDD process in vivo . A: Diagram of the animal procedure for this study. B: Grouping information for this section. C: Representative X-rays of each group of rats. Statistical graph demonstrating the disc height index (DHI) changes for L4/5 in each group of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). D: HE staining exhibiting pathological changes of CEP, NP, and AP in IVD in each group of rats (N = 6). Scale bar: 500 μm. E: EdU staining was used to detect cell proliferation in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. F: TUNEL (white light) staining exhibiting TUNEL-positive cells in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. G: The effect of hsa_circ_0101645 on the protein levels of Collagen Ⅱ, Aggrecan, MMP-3 and MMP-13 in IVD was observed by IHC staining (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 100 μm. H-I: The expression of hsa_circ_0101645 (H) and miR-1304-5p (I) in each group of IVD tissues (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). J-K: Changes in expression of apoptosis ( J; Caspase 3, Bcl-2 and Bax) and autophagy markers ( K; LC3B, Beclin and P62) in IVD tissues (N = 3) (One-way ANOVA test with Tukey's multiple comparisons test or Kruskal-Wallis test with Dunn's multiple comparisons test). ∗ indicates P < 0.05.

Article Snippet: Sections were then incubated with primary antibodies against Collagen II (28459-1-AP; 1:200; Proteintech, USA), Aggrecan (13880-1-AP; 1:100; Proteintech), MMP-3 (17873-1-AP; 1:200; Proteintech), and MMP-13 (18165-1-AP; 1:100; Proteintech), followed by the corresponding secondary antibodies.

Techniques: In Vivo, Staining, TUNEL Assay, Immunohistochemistry, Expressing